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Assessing the in vitro Binding Affinity of Protein–RNA Interactions Using an RNA Pull-down Technique

Anand Chopra, Feras Balbous, Kyle K. Biggar

Infection of the Developing Central Nervous System of Drosophila by Mammalian Eukaryotic and Prokaryotic Pathogens

Billel Benmimoun, Bente Winkler, Pauline Spéder

Conditioned Lick Suppression: Assessing Contextual, Cued, and Context-cue Compound Fear Responses Independently of Locomotor Activity in Mice

Youcef Bouchekioua, Naoya Nishitani, Yu Ohmura

An in vitro Blood-brain Barrier Model to Study the Penetration of Nanoparticles

Sezin Aday, Wen Li, Jeffrey M. Karp, Nitin Joshi

Labeling Endogenous Proteins Using CRISPR-mediated Insertion of Exon (CRISPIE)

Evan A. Wilson, Tianyi Mao, Haining Zhong

Whole-genome Methylation Analysis of APOBEC Enzyme-converted DNA (~5 kb) by Nanopore Sequencing

Suzuko Zaha, Yoshitaka Sakamoto, Satoi Nagasawa, Sumio Sugano, Ayako Suzuki, Yutaka Suzuki, Masahide Seki

Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe

Farzane Sivandzade, Aditya Bhalerao, Luca Cucullo

Detection and Analysis of Circular RNAs by RT-PCR

Amaresh C Panda, Myriam Gorospe

DQ-Red BSA Trafficking Assay in Cultured Cells to Assess Cargo Delivery to Lysosomes

Rituraj Marwaha, Mahak Sharma

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Gregory Finnigan

Professor at Kansas State University

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Murugappan Sathappa

Research scientist at the Broad Institute of MIT and Harvard

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Associate specialist at UCSF

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【Bio-protocol 实验方法解读】北京林业大学李中海课题组详细介绍了一种简单有效的方法来制备和转染毛白杨叶肉原生质体，也证实了该方法同样适用于其它木本植物如桉树（巨尾桉、尾巨桉）叶肉原生质体的游离与转化，供研究人员参考和应用。

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